Characterization of theHansenula polymorpha PUR7gene and its use as selectable marker for targeted chromosomal integration

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Characterization of the Hansenula polymorpha PUR7 gene and its use as selectable marker for targeted chromosomal integration.

The Hansenula polymorpha genes encoding the putative functional homologs of the enzymes involved in the seventh and eighth step in purine biosynthesis, HpPUR7 and HpPUR8, were cloned and sequenced. An overexpression vector designated pHIPA4 was constructed, which contains the HpPUR7 gene as selectable marker and allows expression of genes of interest via the strong, inducible alcohol oxidase pr...

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ژورنال

عنوان ژورنال: FEMS Yeast Research

سال: 2002

ISSN: 1567-1356,1567-1364

DOI: 10.1111/j.1567-1364.2002.tb00064.x